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The 1-Step 2X Multiplex RT-PCR Master Mix is used for real-time qualitative and quantitative multiplex RT-PCR amplifications with TaqMan probes for up to four templates
Internal Control (IC) Acceptance criteria (Ct thresholds) Typical interpretation: FAM positive cutoff Ct <40
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appropriate amount of cells should be added to a new T25 cell culture flask after passage
particularly within theGUS (β-glucuronidase) reporter system
MycoSHENTEK™ Mycoplasma DNA Detection Kit (2G) package size:50 Reactions The 1-Step 2X Multiplex RT-PCRDetection Method qPCR Assay Time Refer to manual Storage Temperature 20C (stable for 24 months) Product Description MycoSHENTEK Mycoplasma DNA Detection Kit (2G) is a qualitative qPCR based assay for detection of Mycoplasma, Spiroplasma and Acholeplasma contamination in master cell banks, working cell banks, virus seed stocks and cell culturederived products. The kit is validated according to USP <63>, EP 2. 6. 7 and JP XVIII, with a stated limit of